Selection of reliable reference genes for RT-qPCR analysis.

@article{Hellemans2014SelectionOR,
  title={Selection of reliable reference genes for RT-qPCR analysis.},
  author={Jan Hellemans and Jo Vandesompele},
  journal={Methods in molecular biology},
  year={2014},
  volume={1160},
  pages={19-26}
}
Reference genes have become the method of choice for normalization of qPCR data. It has been demonstrated in many studies that reference gene validation is essential to ensure accurate and reliable results. This chapter describes how a pilot study can be set up to identify the best set of reference genes to be used for normalization of qPCR data. The data from such a pilot study should be analyzed with dedicated algorithms such as geNorm to rank genes according to their stability--a measure for… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 3 times over the past 90 days. VIEW TWEETS

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 32 extracted citations

Similar Papers

Loading similar papers…