Selection of reliable reference genes for RT-qPCR analysis.

  title={Selection of reliable reference genes for RT-qPCR analysis.},
  author={Jan Hellemans and Jo Vandesompele},
  journal={Methods in molecular biology},
Reference genes have become the method of choice for normalization of qPCR data. It has been demonstrated in many studies that reference gene validation is essential to ensure accurate and reliable results. This chapter describes how a pilot study can be set up to identify the best set of reference genes to be used for normalization of qPCR data. The data from such a pilot study should be analyzed with dedicated algorithms such as geNorm to rank genes according to their stability--a measure for… CONTINUE READING
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