Selection of reference genes for reverse transcription quantitative real-time PCR normalization in black rockfish (Sebastes schlegeli).

@article{Liman2013SelectionOR,
  title={Selection of reference genes for reverse transcription quantitative real-time PCR normalization in black rockfish (Sebastes schlegeli).},
  author={M. G. Liman and Wang Wenji and Liu Conghui and Yu Haiyang and Wang Zhigang and Wang Xubo and Qi Jie and Zhang Quanqi},
  journal={Marine genomics},
  year={2013},
  volume={11},
  pages={67-73}
}
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a technique widely used for quantification of mRNA transcription. Data normalization is an indispensable process for RT-qPCR and reference genes are most commonly used to normalize RT-qPCR and to reduce possible errors generated in the quantification of genes among several proposed methods. To date, RT-qPCR has been used in terms of gene expression studies in black rockfish (Sebastes schlegeli) but the majority… CONTINUE READING