Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions

@article{Wang2014SelectionOR,
  title={Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions},
  author={Zheng Wang and Yan Chen and Hedi Fang and Haifeng Shi and Keping Chen and Zhiyan Zhang and Xiaoli Tan},
  journal={Molecular Genetics and Genomics},
  year={2014},
  volume={289},
  pages={1023-1035}
}
Data normalization is essential for reliable output of quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) assays, as the unsuitable choice of reference gene(s), whose expression might be influenced by exogenous treatments in plant tissues, could cause misinterpretation of results. To date, no systematic studies on reference genes have been performed in stressed Brassica napus. In this study, we investigated the expression variations of nine candidate reference… CONTINUE READING

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