Selection of reference genes for quantitative real-time reverse transcription-polymerase chain reaction in concanavalin A-induced hepatitis model.

@article{Shi2010SelectionOR,
  title={Selection of reference genes for quantitative real-time reverse transcription-polymerase chain reaction in concanavalin A-induced hepatitis model.},
  author={Guojun Shi and Zhijian Zhang and Dechun Feng and Yan Xu and Yan Lu and Jiqiu Wang and Jingjing Jiang and Zhiguo Zhang and Xiaoying Li and Guang Ning},
  journal={Analytical biochemistry},
  year={2010},
  volume={401 1},
  pages={81-90}
}
Quantitative real-time reverse transcription-polymerase chain reaction (Q-PCR) has become an indispensable technique for accurate determination of gene expression in various samples. In mice, intravenous injection of concanavalin A (ConA) leads to acute hepatitis and liver injury. Functional studies based on this model have provided insights for understanding the mechanisms of liver injury. However, no data have been reported to validate reference genes during the progression of ConA-induced… CONTINUE READING
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