Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce

@article{Sgamma2016SelectionOR,
  title={Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce},
  author={Tiziana Sgamma and Judith Pape and Andrea Juliet Massiah and Stephen Jackson},
  journal={Plant Methods},
  year={2016},
  volume={12},
  pages={1-9}
}
Real-time quantitative polymerase chain reaction (RT-qPCR) analysis is a low cost and sensitive technique that is widely used to measure levels of gene expression. Selecting and validating appropriate reference genes for normalising target gene expression should be the first step in any expression study to avoid inaccurate results. In this study, ten candidate genes were tested for their suitability for use as reference genes in diurnal and developmental timecourse experiments in lettuce. The… CONTINUE READING
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