Selection of recombinant vaccinia viruses (Tian Tan strain) expressing hepatitis B virus surface antigen by using beta-galactosidase as a marker.

@article{Liu1990SelectionOR,
  title={Selection of recombinant vaccinia viruses (Tian Tan strain) expressing hepatitis B virus surface antigen by using beta-galactosidase as a marker.},
  author={G. Q. Liu and Xin Cao and Jia-Ying Zhu and G F Ren and Huaijun Michael Li and Y X Xie and Weiping Peng},
  journal={Science in China. Series B, Chemistry, life sciences & earth sciences},
  year={1990},
  volume={33 2},
  pages={188-97}
}
We constructed a plasmid that contains a small piece of DNA with two vaccinia promoters running in opposite directions--a promoter from a late gene encoding an 11 K polypeptide (P11) and a promoter from an early gene encoding 25K (P25). These promoters were isolated from the Tian Tan strain of vaccinia virus and were flanked by the thymidine kinase (TK) sequence of the same virus. Genes encoding the hepatitis B virus surface antigen (HBsAg) and the Escherichia coli beta-galactosidase (LacZ… CONTINUE READING