Selection of promoters for gene transfer into fish.

  title={Selection of promoters for gene transfer into fish.},
  author={Boaz Moav and Z X Liu and Y Groll and Perry B. Hackett},
  journal={Molecular marine biology and biotechnology},
  volume={1 4-5},
A variety of gene constructs containing carp beta-actin regulatory sequences were tested for their ability to drive transient expression of the chloramphenicol acetyltransferase reporter gene in 3 fish cell lines: carp epithelial cells (EPC), rainbow trout hepatoma cells (RTH149), and rainbow trout fibroblasts (RTG2). The constructs showed a wide variation in their levels of expression, and there were significant differences in the effects of transcriptional elements in the 3 cell lines… CONTINUE READING
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