Selection of phages that inhibit vWF interaction with collagen under both static and flow conditions.

Abstract

Phages from a pentadecamer phage display library were selected for binding to vWF by affinity panning. Bound phages were selectively eluted with human collagen type I. After the third round of panning 95% of individual phage clones bound to vWF. The B8-phage inhibited the binding of collagen to vWF with an IC50 of 0.6 x 10(10) phages/ml, and of vWF to… (More)

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Cite this paper

@article{Ulrichts2001SelectionOP, title={Selection of phages that inhibit vWF interaction with collagen under both static and flow conditions.}, author={Hans Ulrichts and Hilde Depraetere and Jol{\'a}n H{\'a}rsfalvi and Hans Deckmyn}, journal={Thrombosis and haemostasis}, year={2001}, volume={86 2}, pages={630-5} }