Selection of phage antibodies by binding affinity. Mimicking affinity maturation.

@article{Hawkins1992SelectionOP,
  title={Selection of phage antibodies by binding affinity. Mimicking affinity maturation.},
  author={Robert Edward Hawkins and Stephen Russell and Greg Winter},
  journal={Journal of molecular biology},
  year={1992},
  volume={226 3},
  pages={889-96}
}
We describe a process, based on display of antibodies on the surface of filamentous bacteriophage, for selecting antibodies either by their affinity for antigen or by their kinetics of dissociation (off-rate) from antigen. For affinity selection, phage are mixed with small amounts of soluble biotinylated antigen (less than 1 microgram) such that the antigen is in excess over phage but with the concentration of antigen lower than the dissociation constant (Kd) of the antibody. Those phage bound… CONTINUE READING

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