Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly

  title={Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly},
  author={Sandra R. Whaley and Douglas S. English and Evelyn L. Hu and Paul F. Barbara and Angela M. Belcher},
In biological systems, organic molecules exert a remarkable level of control over the nucleation and mineral phase of inorganic materials such as calcium carbonate and silica, and over the assembly of crystallites and other nanoscale building blocks into complex structures required for biological function. This ability to direct the assembly of nanoscale components into controlled and sophisticated structures has motivated intense efforts to develop assembly methods that mimic or exploit the… 
Biologically inspired materials for electro-responsive coatings and the photo-oxidation of water
Evolving out of research on biomineralization, a new field devoted to studying the interactions between inorganic materials and proteins is emerging. In natural systems, proteins are responsible for
Discovery of catalytic peptides for inorganic nanocrystal synthesis by a combinatorial phage display approach.
The methodology provides a simple and convenient route to discover a catalytic peptide for ZnO nanocrystal growth at room temperature and the ZP-1 peptide induces non-classical crystallization process which conventional ZnNO synthetic methods cannot match.
Probing the interface between biomolecules and inorganic materials using yeast surface display and genetic engineering.
Although promising for biomimetic materials applications, polypeptides binding inorganic material surfaces and the mechanism of their function have been difficult to characterize. This paper reports
Structure of peptides on metal oxide surfaces probed by NMR.
NMR methods are developed that can be applied to a wide variety of abiotic interfaces to provide an insight into the relationship between the primary sequence of peptides and their functionality at the interface.
Peptide-mediated synthesis of gold nanoparticles: effects of peptide sequence and nature of binding on physicochemical properties.
It is suggested that substantially different Au-peptide interfaces are generated using different peptide sequences, even when these sequences have similar binding affinity, and variations in both nanoparticle surface reconstruction and peptide configuration could be used to program specific functionality into the peptide-capped nanomaterials.
Genetically engineered gold-binding polypeptides: structure prediction and molecular dynamics
This paper presents structure predictions for gold-binding protein sequences, originally selected by combinatorial techniques, and the dynamics of the binding process and the effects of surface topography on the specificity of protein binding.
Peptide-directed co-assembly of nanoprobes on multimaterial patterned solid surfaces
Biocombinatorially selected solid-binding peptides, through their unique material affinity and selectivity, are a promising platform for building up complex hierarchical assemblies of nanoscale
Biological scaffolds for the peptide-directed assembly of nanoscale materials and devices
The utilization of biological factors in the design, synthesis and fabrication of nano-scaled materials and devices presents novel, large scale solutions for the realization of future technologies.
Biointerface Structural Effects on the Properties and Applications of Bioinspired Peptide-Based Nanomaterials.
How the versatility of this peptide-mediated approach for the growth, organization, and activation of nanomaterials could be more widely expanded via the elucidation of biointerfacial structure/property relationships is illustrated.
Controlling self-assembly of engineered peptides on graphite by rational mutation.
It is demonstrated here that short dodecapeptides selected by phage display are capable of self-assembly on graphite and form long-range-ordered biomolecular nanostructures with uniformly displayed residues.


Organization of 'nanocrystal molecules' using DNA
A strategy for the synthesis of 'nanocrystal molecules', in which discrete numbers of gold nanocrystals are organized into spatially defined structures based on Watson-Crick base-pairing interactions is described.
A DNA-based method for rationally assembling nanoparticles into macroscopic materials
A method for assembling colloidal gold nanoparticles rationally and reversibly into macroscopic aggregates by using the specificity of DNA interactions to direct the interactions between particles of different size and composition is described.
Silicatein filaments and subunits from a marine sponge direct the polymerization of silica and silicones in vitro.
  • J. Cha, K. Shimizu, +4 authors D. Morse
  • Chemistry, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1999
It is shown that the protein filaments and their constituent subunits comprising the axial cores ofsilica spicules in a marine sponge chemically and spatially direct the polymerization of silica and silicone polymer networks from the corresponding alkoxide substrates in vitro, under conditions in which such syntheses otherwise require either an acid or base catalyst.
Control of crystal phase switching and orientation by soluble mollusc-shell proteins
IN the initial stages of the biomineralization of abalone shells, a primer layer of oriented calcite crystals grows on a nucleating protein sheet1,2. The deposition of this primer is followed by an
Semiconductor nanocrystals covalently bound to metal surfaces with self-assembled monolayers
A method is described for attaching semiconductor nanocrystals to metal surfaces using self-assembled difunctional organic monolayers as bridge compounds. Three different techniques are presented.
Metal-recognition by repeating polypeptides
Repeating polypeptides able to bind to metallic gold or chromium were selected and were found to retain their binding properties when freed from the protein used to select them.
Organization of Inorganic Nanoparticles Using Biotin−Streptavidin Connectors
Organizing inorganic matter at the nanometer and mesoscopic length scale represents a new challenge for materials chemistry. One potential strategy involves the use of biomolecular recognition in the
Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor.
  • S. Brown
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1992
Results of this genetic analysis indicate that in some isolates, part of the binding site is encoded by flanking vector sequences, which may prove generally useful for identifying protein sequences able to recognize specific surfaces.
Control of Aragonite or Calcite Polymorphism by Mollusk Shell Macromolecules
Many mineralizing organisms selectively form either calcite or aragonite, two polymorphs of calcium carbonate with very similar crystalline structures. Understanding how these organisms achieve this
Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.
Fusion-phage vectors that accept foreign DNA inserts with little effect on phage function are introduced; affinity purification of virions bearing a target determinant from a 10(8)-fold excess of phage not bearing the determinant is described, using minute amounts of antibody.