Selection of peptide entry motifs by bacterial surface display.

@article{Taschner2002SelectionOP,
  title={Selection of peptide entry motifs by bacterial surface display.},
  author={Sabine Taschner and Andreas Meinke and Alexander von Gabain and Aoife P Boyd},
  journal={The Biochemical journal},
  year={2002},
  volume={367 Pt 2},
  pages={
          393-402
        }
}
Surface display technologies have been established previously to select peptides and polypeptides that interact with purified immobilized ligands. In the present study, we designed and implemented a surface display-based technique to identify novel peptide motifs that mediate entry into eukaryotic cells. An Escherichia coli library expressing surface-displayed peptides was combined with eukaryotic cells and the gentamicin protection assay was performed to select recombinant E. coli, which were… CONTINUE READING

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