Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography.

@article{Patwardhan1997SelectionOO,
  title={Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography.},
  author={Anand V. Patwardhan and G N Goud and Richard R Koepsel and Mohammad M. Ataai},
  journal={Journal of chromatography. A},
  year={1997},
  volume={787 1-2},
  pages={91-100}
}
Immobilized metal affinity chromatography (IMAC) is a versatile tool for the purification of proteins with affinity for immobilized metals. Moreover, this technique has also been used for the separation of proteins that do not exhibit significant metal affinity in the native form, by their fusion to a short metal-binding peptide (a tail), most commonly, a sequence consisting of six adjacent histidine residues (His6). A phage-displayed random hexamer library is used to select for peptides with… CONTINUE READING

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