Selection of membrane IgM- variants from a mIgM+ murine B lymphoma cell: problems and solutions.

@article{AndrewsWagner1983SelectionOM,
  title={Selection of membrane IgM- variants from a mIgM+ murine B lymphoma cell: problems and solutions.},
  author={Rebecca Andrews-Wagner and Carol Hopkins Sibley},
  journal={Somatic cell genetics},
  year={1983},
  volume={9 1},
  pages={43-54}
}
We have used antibody-mediated complement killing to isolate membrane IgM-negative (mIgM-) variants from the mIgM+ murine B cell lymphoma, WEHI 279.1. This procedure has been used previously to select variants which lack expression of other cell-surface antigens on lymphoid cells. In those experiments, multiple rounds of selection have often been required for selection of the negative variants. We found that many cycles of selection produced very few variants and that those isolated had reduced… CONTINUE READING