Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer.

@article{Bhojwani2007SelectionOD,
  title={Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer.},
  author={Sanjay Bhojwani and Hannelore Alm and Helmut Torner and Wilhelm Kanitz and Ralf Poehland},
  journal={Theriogenology},
  year={2007},
  volume={67 2},
  pages={341-5}
}
The aim of the present investigation was to study the effect of oocyte selection on the efficiency of bovine nuclear transfer in terms of increased blastocyst production. For this purpose, prior to in vitro maturation (IVM), oocytes were selected for their developmental competence on the basis of glucose-6-phosphate dehydrogenase (G6PDH) activity indicated by brilliant cresyl blue (BCB) staining. It has been hypothesized that growing oocytes have a higher level of active G6PDH in comparison to… CONTINUE READING