Selection of candidate housekeeping controls in tomato plants using EST data.

@article{Coker2003SelectionOC,
  title={Selection of candidate housekeeping controls in tomato plants using EST data.},
  author={Jeffrey Scott Coker and Eric Davies},
  journal={BioTechniques},
  year={2003},
  volume={35 4},
  pages={
          740-2, 744, 746 passim
        }
}
Because the expression levels of housekeeping genes are relatively constant in most tissues, they are often useful controls when quantifying gene expression. We present an analytical method for identifying candidate housekeeping controls using expressed sequence tags (ESTs) from The Institute for Genomic Research Tomato Gene Index. We found relative expression levels for a collection of 127 transcripts and calculated the percentage of cDNA libraries that had expression levels (for a given… CONTINUE READING
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