Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber.

@article{Wan2010SelectionOA,
  title={Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber.},
  author={Hongjian Wan and Zhenguo Zhao and Chuntao Qian and Yihu Sui and Ahmed Abbas Malik and Jinfeng Chen},
  journal={Analytical biochemistry},
  year={2010},
  volume={399 2},
  pages={257-61}
}
Quantitative real-time polymerase chain reaction (QRT-PCR) has become one of the most widely used methods for gene expression analysis. However, the expression profile of a target gene may be misinterpreted due to unstable expression of the reference genes under different experimental conditions. Thus, a systematic evaluation of these reference genes is necessary before experiments are performed. In this study, 10 putative reference genes were chosen for identifying expression stability using… CONTINUE READING
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