Selection of an Escherichia coli host that expresses mutant forms of Mycobacterium tuberculosis 2-trans enoyl-ACP(CoA) reductase and 3-ketoacyl-ACP(CoA) reductase enzymes.

@article{Poletto2004SelectionOA,
  title={Selection of an Escherichia coli host that expresses mutant forms of Mycobacterium tuberculosis 2-trans enoyl-ACP(CoA) reductase and 3-ketoacyl-ACP(CoA) reductase enzymes.},
  author={Simone S Poletto and Isabel Os{\'o}rio da Fonseca and Luiz Pedro S de Carvalho and Luiz Augusto Basso and Di{\'o}genes Santiago Santos},
  journal={Protein expression and purification},
  year={2004},
  volume={34 1},
  pages={118-25}
}
Tuberculosis (TB) still remains a worldwide health concern. Efforts to understand the complex biology of Mycobacterium tuberculosis, the causative agent of TB, are important for new antitubercular drug development. Despite the completion of the genome sequence and the development of new genetic tools to manipulate this organism, the availability of sufficient amounts of mycobacterial proteins still remains an essential and laborious step to study the biochemical features of this pathogen. The… CONTINUE READING