Selection of an Appropriate Protein Extraction Method to Study the Phosphoproteome of Maize Photosynthetic Tissue

Abstract

Often plant tissues are recalcitrant and, due to that, methods relying on protein precipitation, such as TCA/acetone precipitation and phenol extraction, are usually the methods of choice for protein extraction in plant proteomic studies. However, the addition of precipitation steps to protein extraction methods may negatively impact protein recovery, due… (More)
DOI: 10.1371/journal.pone.0164387

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Cite this paper

@inproceedings{Lus2016SelectionOA, title={Selection of an Appropriate Protein Extraction Method to Study the Phosphoproteome of Maize Photosynthetic Tissue}, author={In{\^e}s vaz Lu{\'i}s and Bruno M. Alexandre and M Margarida Oliveira and Isabel A Abreu}, booktitle={PloS one}, year={2016} }