Selection of alternative 5' splice sites: role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1.

@article{Eperon2000SelectionOA,
  title={Selection of alternative 5' splice sites: role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1.},
  author={I. C. Eperon and Olga V. Makarova and Akila Mayeda and Stephen H. Munroe and Javier F C{\'a}ceres and Daniel Geoffrey Hayward and Adrian R. Krainer},
  journal={Molecular and cellular biology},
  year={2000},
  volume={20 22},
  pages={8303-18}
}
The first component known to recognize and discriminate among potential 5' splice sites (5'SSs) in pre-mRNA is the U1 snRNP. However, the relative levels of U1 snRNP binding to alternative 5'SSs do not necessarily determine the splicing outcome. Strikingly, SF2/ASF, one of the essential SR protein-splicing factors, causes a dose-dependent shift in splicing to a downstream (intron-proximal) site, and yet it increases U1 snRNP binding at upstream and downstream sites simultaneously. We show here… CONTINUE READING

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Highly Influential
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