Selection of a pure cerebellar granule cell culture by kainate treatment

@article{Drejer1989SelectionOA,
  title={Selection of a pure cerebellar granule cell culture by kainate treatment},
  author={J\orgen Drejer and Arne Schousboe},
  journal={Neurochemical Research},
  year={1989},
  volume={14},
  pages={751-754}
}
Chronic exposure of dissociated cerebellar cultures to 50μM kainate results in a complete loss of [3H]-GABA release which is a marker of GABAergic interneurons. No loss of granule cells was found and the glutamatergic nature of the granule cells appeared unaltered by the kainate treatment, since evoked release of [3H]-d-aspartate was maintained after kainate exposure. Glial cells in such cultures are virtually eliminated by treatment with an antimitotic such as cytarabin. In consequence a pure… CONTINUE READING

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