Selection of T-DNA-tagged male and female gametophytic mutants by segregation distortion in Arabidopsis.

  title={Selection of T-DNA-tagged male and female gametophytic mutants by segregation distortion in Arabidopsis.},
  author={Ross Howden and Soon Ki Park and James M. Moore and James Orme and Ueli Grossniklaus and David Twell},
  volume={149 2},
As a strategy for the identification of T-DNA-tagged gametophytic mutants, we have used T-DNA insertional mutagenesis based on screening for distorted segregation ratios by antibiotic selection. Screening of approximately 1000 transgenic Arabidopsis families led to the isolation of eight lines showing reproducible segregation ratios of approximately 1:1, suggesting that these lines are putative gametophytic mutants caused by T-DNA insertion at a single locus. Genetic analysis of T-DNA… CONTINUE READING
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