Selection of Shine-Dalgarno sequences in plastids

  title={Selection of Shine-Dalgarno sequences in plastids},
  author={Oliver Drechsel and Ralph Bock},
  booktitle={Nucleic acids research},
Like bacterial genes, most plastid (chloroplast) genes are arranged in operons and transcribed as polycistronic mRNAs. Plastid protein biosynthesis occurs on bacterial-type 70S ribosomes and translation initiation of many (but not all) mRNAs is mediated by Shine-Dalgarno (SD) sequences. To study the mechanisms of SD sequence recognition, we have analyzed translation initiation from mRNAs containing multiple SD sequences. Comparing translational efficiencies of identical transgenic mRNAs in… CONTINUE READING