Selection of Reliable Reference Genes for Gene Expression Studies of a Promising Oilseed Crop, Plukenetia volubilis, by Real-Time Quantitative PCR

@inproceedings{Niu2015SelectionOR,
  title={Selection of Reliable Reference Genes for Gene Expression Studies of a Promising Oilseed Crop, Plukenetia volubilis, by Real-Time Quantitative PCR},
  author={Longjian Niu and Yan-Bin Tao and Mao-Sheng Chen and Qiantang Fu and Chaoqiong Li and Yuling Dong and Xiulan Wang and Huiying He and Zeng-Fu Xu and Marcello Iriti},
  booktitle={International journal of molecular sciences},
  year={2015}
}
Real-time quantitative PCR (RT-qPCR) is a reliable and widely used method for gene expression analysis. The accuracy of the determination of a target gene expression level by RT-qPCR demands the use of appropriate reference genes to normalize the mRNA levels among different samples. However, suitable reference genes for RT-qPCR have not been identified in Sacha inchi (Plukenetia volubilis), a promising oilseed crop known for its polyunsaturated fatty acid (PUFA)-rich seeds. In this study, using… CONTINUE READING
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