Selection of RNA aptamers that are specific and high-affinity ligands of the hepatitis C virus RNA-dependent RNA polymerase.

@article{Biroccio2002SelectionOR,
  title={Selection of RNA aptamers that are specific and high-affinity ligands of the hepatitis C virus RNA-dependent RNA polymerase.},
  author={Antonino Biroccio and J{\"o}rg Hamm and Ilario Incitti and Raffaele De Francesco and Licia Tomei},
  journal={Journal of virology},
  year={2002},
  volume={76 8},
  pages={3688-96}
}
In order to find small RNA molecules that are specific and high-affinity ligands of nonstructural 5B (NS5B) polymerase, we screened by SELEX (systematic evolution of ligands by exponential amplification) a structurally constrained RNA library with an NS5BDeltaC55 enzyme carrying a C-terminal biotinylation sequence. Among the selected clones, two aptamers appeared to be high-affinity ligands of NS5B, with apparent dissociation constants in the low nanomolar range. They share a sequence that can… CONTINUE READING

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