Selection in HAT medium is not a reliable method for the study of reversion from 6-thioguanine resistance to sensitivity.

Abstract

6-Thioguanine-resistant mutants were induced in V79 Chinese hamster cells with 2-aminopurine, ICR-170 and hycanthone. Samples of mutants of different origin were treated with EMS or 1CR-170 and plated in HAT medium for selection of revertants. In the result, a significant fraction of HAT-resistant clones was not 6-thioguanine-sensitive as would have been expected from a genuine reversion to wild type.

Cite this paper

@article{Bonatti1982SelectionIH, title={Selection in HAT medium is not a reliable method for the study of reversion from 6-thioguanine resistance to sensitivity.}, author={Stefano Bonatti and Aldo Di Leonardo and Laura Mariani and R. F. Randazzo and Giulia Sciandrello}, journal={Mutation research}, year={1982}, volume={104 6}, pages={377-81} }