Selection and micropropagation of high artemisinin producing clones of Artemisia annua L.

  title={Selection and micropropagation of high artemisinin producing clones of Artemisia annua L.},
  author={H. M. Elhag and M. M. El-domiaty and Farouk S. El-Feraly and Jaber S. Mossa and Mahmoud M. El-Olemy},
  journal={Phytotherapy Research},
An in vitro micropropagation method has been used to clone Artemisia annua L. plants from axenic seedlings or field grown plants selected on the basis of morphological characteristics during the growing season. Clones were maintained in culture by continuous transfer every 4–6 weeks. The performance of the various clones in the field as well as their contents of artemisinin and related sesquiterpenes were compared with selected field grown plants. An internally standardized reverse‐phase HPLC… Expand
Isolation of high artemisinin-yielding clones of Artemisia annua☆
The artemis inin profiles of the micropropagated progenies at vegetative, preflowering and full bloom stages indicated that while highest artemisinin accumulation occurred at full bloom stage, the art Artemisinin content at preflowered stage was positively correlated with that at full Bloom stage. Expand
In Vitro Micropropagation and Flowering in Artemisia annua
Since artemisinin estimation is correlative to flowering, the results would facilitate better understanding of biosynthesis of this drug in vitro. Expand
Effects of Environmental Factors on Growth and Artemisinin Content of Artemisia annua L.
This study indicated that the variations observed in plant growth and artemisinin contents were due to temperature effects because the two selected clones were genetically homogenous. Expand
Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L.
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Toward the production of artemisinin through tissue culture: Determining nutrient-hormone combinations suitable for cell suspension cultures
It was determined that friable callus formed when combinations of NAA with kinetin or 2,4-D and BA were used with B5 medium, and no single combination of concentrations of auxin and cytokinin seemed to be “ideal” for producing friablecallus. Expand
Impact of plant growth regulators (PGRs) on callogenesis and artemisinin content in Artemisia annua L. plants
Received 5 May 2013; revised 30 October 2013; accepted 12 November 2013 Callus cultures were initiated from leaf explants of Artemisia annua L. plants for artemisinin production using Murashige andExpand
Efficient Somatic Embryogenesis and Organogenesis of Self-Pollination Artemisia annua Progeny and Artemisinin Formation in Regenerated Plants
The results show the highly efficient regeneration capacity of self-pollination A. annua plants that can form a new platform to enhance the understanding of artemisinin biosynthesis and metabolic engineering. Expand
Seasonal and Differential Sesquiterpene Accumulation in Artemisia annua Suggest Selection Based on Both Artemisinin and Dihydroartemisinic Acid may Increase Artemisinin in planta
The data suggest that different chemotypes, high in ART and DHAA, have complementary pathways, while competing with AA, and that crossing plants high in Artemisinin andDHAA may generate hybrids with higher ART than currently available in commercial germplasms. Expand
Distinctive morphological characteristics contribute to the identification of Artemisia annua L. germplasms with high yield and high artemisinin content
Both plant height and stem bottom diameter had the most important positive influence on artemisinin content of the leaves and herb yield, and can help to increase breeding efficiency for getting new A. annua L. cultivars with high herbal yield and high artemis inin content. Expand


Formation of Artemisinin in Artemisia annua During One Vegetation Period*,1.
In order to investigate the possibility of isolating greater amounts of the antimalarial compound artemisinin (quinghaosu), plants of ARTEMISIA ANNUA were cultivated and analysed at different stages of development, and two hormone-type growth regulators were tested on A. ANNUA strain 811. Expand
The Production of Artemisinin in Artemisia annua L. Tissue Cultures
The genus Artemisia is one of the largest and is the most geographically distributed of the 102 genera in the Anthemideae tribe of the compositae family and efforts are being made to select high-content artemisinin plants from field-grown Artemisia plants, although the selection criteria presently used are not well defined. Expand
Germplasm Variation in Artemisinin Content of Artemism annua Using an Alternative Method of Artemisinin Analysis from Crude Plant Extracts
Artemisinin content was evaluated in a germplasm collection of Artemisia annua using an HPLC-EC detector with a glassy carbon electrode and the highest artemisin in the leaves from the top 50-cm portion of the plant was found. Expand
On the possible role of qinghao acid in the biosynthesis of artemisinin
Abstract Artemisinin (qinghaosu), a seco -sesquiterpene peroxide, is the clinically established antimalarial principle isolated from the leaves of the Chinese medicinal herb, Artemisia annua . RecentExpand
Qinghaosu (artemisinin): an antimalarial drug from China
Derivatives of QHS, such as dihydroqinghaosu, artemether, and the water-soluble sodium artesunate, appear to be more potent than QHS itself, and offer promise as a totally new class of antimalarials. Expand
A revised medium for rapid growth and bio assays with tobacco tissue cultures
In experiments with tobacco tissue cultured on White's modified medium supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Expand
Nutrient requirements of suspension cultures of soybean root cells.
Abstract The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyaceticExpand
Reductive electrochemical HPLC assay for artemisinin (qinghaosu).
An electrochemical HPLC detection procedure has been developed that provides a sensitive, selective, and rapid assay for artemisinin in crude plant extracts. Expand
Conversion of artemisinin to artemisitene
Artemisinin, a potent antimalarial sesquiterpene endoperoxide, was converted via a photochemical route to its dehydro analogue, artemisitene
Effect of Point Polarity on Conduction and Burst Current in an Inhomogeneous Field in Transformer Oil
Abstract This paper presents a study of the effect of point cathode and point anode on conduction and burst currents under high fields in transformer oil from a transformer in service. It was foundExpand