Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines.


Variants of malignant RAW117 lymphosarcoma cell lines were selected by sequential nonadherence to polystyreneImmobilized lectlns. Parental murine lymphosarcoma line RAW117-P grew In suspension and was rapidly bound by Immobilized concanavalin A (Con A), Ricinus communis agglutinin I (RCAI), peanut agglutinin (PNA), or wheat germ agglutinin (WGA). After attachment of most cells to lectin-substituted petri dishes, the unattached cells «1%) were removed by aspiration and subcultured. Selection by nonadherence to Immobilized lectlns was repeated seven to ten times, and the resulting cell lines were tested in BALB/c mice to determine their malignant potentials. Parental line RAW117-P formed few (median, 0; range, 0-20) liver tumor colonies per 5x10' Iv Injected cells after 14 days, whereas a variant line selected ten times on Immobilized Con A (RAW117P Con d'o,) formed a median of 95 (range, 9->200) liver colonies. RAW117-P cells selected by nonadherence on immobilized WGA, PNA, or RCAI showed no increase in liver tumor formation compared to the cells of the parental line. In contrast, RAW117-H10, a variant line selected ten times In vivo for liver colonization, formed more than 200 liver tumor colonies, and selection eight times on Immobilized WGA yielded a line (RAW117-H10 WGA"8) that formed a median of only 1 (range, 0-4) tumor colony. Variants of the RAW117-H10 line selected on Immobilized RCAI, Con A, or PNA still formed more than 200 liver tumor colonies. 1251-labeled lectin-binding studies Indicated decreased lectin-binding sites on the In vivo and some of the immobilized lectin-selected lines. Autoradlographs of '''I-labeled Con A-stained sodium dodecyl sulfate polyacrylamide gels showed a decrease In the major Con A-staining band (mol wt, =70,000) In the in vivo and Immobilized Con A-selected lines. These studies Indicated that alterations In cell surface lectin receptors could modify in vivo malignant behavior.-JNCI 64:

4 Figures and Tables


Citations per Year

509 Citations

Semantic Scholar estimates that this publication has 509 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Reading1980SelectionAI, title={Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines.}, author={Chris L. Reading and P N Belloni and GL Nicolson}, journal={Journal of the National Cancer Institute}, year={1980}, volume={64 5}, pages={1241-9} }