Selection and identification of single domain antibody fragments from camel heavy-chain antibodies.

@article{Ghahroudi1997SelectionAI,
  title={Selection and identification of single domain antibody fragments from camel heavy-chain antibodies.},
  author={Mehdi Arbabi Ghahroudi and Aline I. Desmyter and Lode Wyns and Raymond Hamers and Serge Muyldermans},
  journal={FEBS letters},
  year={1997},
  volume={414 3},
  pages={
          521-6
        }
}
Functional heavy-chain gamma-immunoglobulins lacking light chains occur naturally in Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire of the variable domains of its heavy-chain antibodies and panning, leading to the successful identification of minimum sized antigen binders. The recombinant binders are expressed well in E. coli, extremely stable, highly soluble, and react specifically and with high affinity to the antigens. This approach can be viewed as… CONTINUE READING
BETA

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 272 CITATIONS

Application of camelid heavy-chain variable domains (VHHs) in prevention and treatment of bacterial and viral infections.

  • International reviews of immunology
  • 2018
VIEW 4 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

Nanobody Technology for Mycotoxin Detection: Current Status and Prospects

  • Toxins
  • 2018
VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

In Vivo Neutralization of Botulinum Neurotoxins Serotype E with Heavy-chain Camelid Antibodies (VHH)

Hamid Bakherad, Seyed Latif Mousavi Gargari, +5 authors Hamed Zare
  • Molecular biotechnology
  • 2013
VIEW 4 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

Single-domain Antibody Inhibitors of Clostridium difficile Toxins

VIEW 11 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Potent enzyme inhibitors derived from dromedary heavy-chain antibodies.

VIEW 6 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

1998
2018

CITATION STATISTICS

  • 21 Highly Influenced Citations

  • Averaged 22 Citations per year over the last 3 years

  • 2% Increase in citations per year in 2018 over 2017

Similar Papers

Loading similar papers…