Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

@article{Frankel1989SelectionAC,
  title={Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.},
  author={Arthur Edward Frankel and David M. Schlossman and Philip C. Welsh and Andrew A. Hertler and David Withers and S P Johnston},
  journal={Molecular and cellular biology},
  year={1989},
  volume={9 2},
  pages={415-20}
}
A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the toxin. A number of mutant alleles which encoded cross-reactive material were sequenced. Eight of the first nine mutant RTAs studied showed single-amino-acid changes involving… CONTINUE READING

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