Selection and Evaluation of Reference Genes for Expression Studies with Quantitative PCR in the Model Fungus Neurospora crassa under Different Environmental Conditions in Continuous Culture

@inproceedings{Cusick2014SelectionAE,
  title={Selection and Evaluation of Reference Genes for Expression Studies with Quantitative PCR in the Model Fungus Neurospora crassa under Different Environmental Conditions in Continuous Culture},
  author={Kathleen D. Cusick and Lisa A. Fitzgerald and Russell K Pirlo and Allison L. Cockrell and Emily R. Petersen and Justin C. Biffinger},
  booktitle={PloS one},
  year={2014}
}
Neurospora crassa has served as a model organism for studying circadian pathways and more recently has gained attention in the biofuel industry due to its enhanced capacity for cellulase production. However, in order to optimize N. crassa for biotechnological applications, metabolic pathways during growth under different environmental conditions must be addressed. Reverse-transcription quantitative PCR (RT-qPCR) is a technique that provides a high-throughput platform from which to measure the… CONTINUE READING
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