Selection and Characterization of Dunaliella salina Mutants Defective in Haloadaptation.

@article{Chitlaru1989SelectionAC,
  title={Selection and Characterization of Dunaliella salina Mutants Defective in Haloadaptation.},
  author={Edith Chitlaru and Uri Pick},
  journal={Plant physiology},
  year={1989},
  volume={91 2},
  pages={
          788-94
        }
}
A technique for selection of Dunaliella mutants defective in their capacity to recover from osmotic shocks has been developed. The selection is based on physical separation of mutants on density gradients. This technique takes advantage of the fact that Dunaliella cells, when exposed to osmotic shocks, initially change volume and density due to water gain or loss and subsequently recover their volume and density by readjusting their intracellular glycerol. Eight mutants that do not recover… CONTINUE READING

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