Selecting folded proteins from a library of secondary structural elements.

@article{Graziano2008SelectingFP,
  title={Selecting folded proteins from a library of secondary structural elements.},
  author={James J Graziano and Wenshe R Liu and Roshan Perera and Bernhard H. Geierstanger and Scott A. Lesley and Peter G. Schultz},
  journal={Journal of the American Chemical Society},
  year={2008},
  volume={130 1},
  pages={176-85}
}
A protein evolution strategy is described by which double-stranded DNA fragments encoding defined Escherichia coli protein secondary structural elements (alpha-helices, beta-strands, and loops) are assembled semirandomly into sequences comprised of as many as 800 amino acid residues. A library of novel polypeptides generated from this system was inserted into an enhanced green fluorescent protein (EGFP) fusion vector. Library members were screened by fluorescence activated cell sorting (FACS… CONTINUE READING

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