Selected reaction monitoring (SRM) mass spectrometry without isotope labeling can be used for rapid protein quantification.

@article{Zhi2011SelectedRM,
  title={Selected reaction monitoring (SRM) mass spectrometry without isotope labeling can be used for rapid protein quantification.},
  author={Wenbo Zhi and Meiyao Wang and Jin-Xiong She},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2011},
  volume={25 11},
  pages={1583-8}
}
The validation of putative biomarker candidates has become the major bottle-neck in protein biomarker development. Conventional immunoaffinity methods are limited by the availability of antibodies and kits. Here we demonstrate the feasibility of using selected reaction monitoring (SRM) without isotope labeling to achieve fast and reproducible quantification of serum proteins. The SRM/MRM assays for three standard serum proteins, including ceruloplasmin (CP), serum aymloid A (SAA) and sex… CONTINUE READING

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