Selectable markers for eukaryotic cells.

  title={Selectable markers for eukaryotic cells.},
  author={R. Vile},
  journal={Methods in molecular biology},
The transfer of DNA sequences into a population of cells can rarely, if ever, be achieved with 100% efficiency. Typically, transfection of cells with the calcium phosphate method will transduce only between 0.1 and 1% of the cells with the sequences of interest (1), although some workers have achieved higher efficiencies (2). If the transferred sequences do not confer a selective growth advantage, it is essential to use selection for transduced cells. The marker gene itself may be the gene of… CONTINUE READING