Segregation of sperm subpopulations in normozoospermic infertile men.

  title={Segregation of sperm subpopulations in normozoospermic infertile men.},
  author={Jayapalraja Thilagavathi and Sundararajan Venkatesh and Rajeev Kumar and Rima Dada},
  journal={Systems biology in reproductive medicine},
  volume={58 6},
Sperm function is essential for fertilization and embryogenesis yet semen contain a heterogeneous population of sperm. This study was designed to evaluate two different sperm populations separated by the density gradient method. Semen from 25 idiopathic normozoospermic infertile men was processed by double density gradient centrifugation and evaluated for sperm present in the 50% (upper) layer and the 90% (lower) layer for reactive oxygen species (ROS), sperm chromatin integrity, and morphology… CONTINUE READING
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