Segregation of molecules at cell division reveals native protein localization

@inproceedings{Landgraf2012SegregationOM,
  title={Segregation of molecules at cell division reveals native protein localization},
  author={Dirk Landgraf and Burak Okumus and P. Monica Chien and Tania A. Baker and Johan Paulsson},
  booktitle={Nature Methods},
  year={2012}
}
We introduce a nonintrusive method exploiting single-cell variability after cell division to validate protein localization. We found that Clp proteases, widely reported to form biologically relevant foci, were uniformly distributed in Escherichia coli cells, and that many commonly used fluorescent proteins caused severe mislocalization when fused to homo-oligomers. Retagging five other reportedly foci-forming proteins with the most monomeric fluorescent protein tested suggests that the foci… CONTINUE READING
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