Segmentation of precursor mass range using "tiling" approach increases peptide identifications for MS1-based label-free quantification.

@article{Vincent2013SegmentationOP,
  title={Segmentation of precursor mass range using "tiling" approach increases peptide identifications for MS1-based label-free quantification.},
  author={Catherine E Vincent and Gregory K Potts and Arne Ulbrich and Michael Scott Westphall and James A. Atwood and Joshua J. Coon and D. Brent Weatherly},
  journal={Analytical chemistry},
  year={2013},
  volume={85 5},
  pages={
          2825-32
        }
}
Label-free quantification is a powerful tool for the measurement of protein abundances by mass spectrometric methods. To maximize quantifiable identifications, MS(1)-based methods must balance the collection of survey scans and fragmentation spectra while maintaining reproducible extracted ion chromatograms (XIC). Here we present a method which increases the depth of proteome coverage over replicate data-dependent experiments without the requirement of additional instrument time or sample… CONTINUE READING

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