Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh

@article{Rizk2014SegmentationAQ,
  title={Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh},
  author={Aur{\'e}lien Rizk and Gr{\'e}gory Paul and Pietro Incardona and Milica Bugarski and Maysam Mansouri and Axel Niemann and Urs Ziegler and Philipp Berger and Ivo F. Sbalzarini},
  journal={Nature Protocols},
  year={2014},
  volume={9},
  pages={586-596}
}
Detection and quantification of fluorescently labeled molecules in subcellular compartments is a key step in the analysis of many cell biological processes. Pixel-wise colocalization analyses, however, are not always suitable, because they do not provide object-specific information, and they are vulnerable to noise and background fluorescence. Here we present a versatile protocol for a method named 'Squassh' (segmentation and quantification of subcellular shapes), which is used for detecting… CONTINUE READING
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