Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

@article{Busche2009SegmentalIL,
  title={Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.},
  author={Alena E L Busche and A. Sesilja Aranko and Mehdi Talebzadeh-Farooji and Frank Bernhard and Volker D{\"o}tsch and Hideo Iwa{\"i}},
  journal={Angewandte Chemie},
  year={2009},
  volume={48 33},
  pages={6128-31}
}
Many proteins, including cellular signaling proteins, cellsurface receptors, and modular enzymes, are constructed from individual domains and connecting linkers. Structural analysis by NMR spectroscopy or by X-ray crystallography often focuses on self-contained domains excised from full-length proteins so as to reduce the molecular weight to a manageable size for high-quality NMR spectra or to improve crystallization by removing disordered regions. Such minimization often neglects functional… CONTINUE READING

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