Seeing the wood through the trees: a review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence.

@article{Billinton2001SeeingTW,
  title={Seeing the wood through the trees: a review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence.},
  author={Nicholas Billinton and Andrew W. Knight},
  journal={Analytical biochemistry},
  year={2001},
  volume={291 2},
  pages={175-97}
}
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has recently leapt to prominence within numerous biological fields. Interest in the use of GFP has grown enormously over the past 5 years. The number of papers concerning GFP held in the Web of Science database, which abstracts the scientific literature (http://wos.mimas.ac.uk), rose from 13 in the period 1981–1994, to over 3400 in the period 1995 to October 2000. This is due to the ability to clone and heterologously… CONTINUE READING
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