Seed-specific expression of a bacterial phosphoenolpyruvate carboxylase in Vicia narbonensis increases protein content and improves carbon economy.

@article{Rolletschek2004SeedspecificEO,
  title={Seed-specific expression of a bacterial phosphoenolpyruvate carboxylase in Vicia narbonensis increases protein content and improves carbon economy.},
  author={Hardy Rolletschek and Ljudmilla Borisjuk and Ruslana I. Radchuk and Manoela Miranda and Ute Heim and U. Wobus and Hans Matthias Weber},
  journal={Plant biotechnology journal},
  year={2004},
  volume={2 3},
  pages={
          211-9
        }
}
An ambitious aim in plant breeding and biotechnology is to increase the protein content of crop seeds used for food and feed. Using an approach to manipulate assimilate partitioning, we succeeded in elevating the protein content in legume seeds up to 50%. Transgenic bean plants were generated which express a Corynebacterium glutamicum phosphoenolpyruvate carboxylase (PEPC) in a seed-specific manner. The bacterial enzyme was not feedback inhibited by malate. Transgenic seeds showed a higher [14C… CONTINUE READING

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