Seed legumains are expressed in stamens and vegetative legumains in seeds of Nicotiana tabacum L.

@article{Zakharov2004SeedLA,
  title={Seed legumains are expressed in stamens and vegetative legumains in seeds of Nicotiana tabacum L.},
  author={Alexander Zakharov and Klaus M{\"u}ntz},
  journal={Journal of experimental botany},
  year={2004},
  volume={55 402},
  pages={
          1593-5
        }
}
Detailed analysis of the expression pattern of seven legumain genes from Nicotiana tabacum L. cv. SNN revealed that it showed neither coincidences with the branches of the phylogenetic tree of legumains nor with their conventional assignment to organs. This agreed well with the fact that, so far, no functional differences could be assigned to the sequence differences reflected in the branches of the tree. 

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References

SHOWING 1-10 OF 13 REFERENCES
Cell Ablation Reveals That Expression from the Phaseolin Promoter Is Confined to Embryogenesis and Microsporogenesis
TLDR
After fertilization of the DT-A-transformed plants with pollen from wild-type tobacco, 50% of the resulting embryos aborted at the heart stage, defining this as the earliest time for phas expression during embryogenesis, and no phas activity could be detected in bean pollen.
The families of papain- and legumain-like cysteine proteinases from embryonic axes and cotyledons of Vicia seeds: developmental patterns, intracellular localization and functions in globulin proteolysis
TLDR
Different functions in molecular maturation and mobilization of storage proteins could be attributed to the various members of the CPR families.
Seed-specific promoters direct gene expression in non-seed tissue.
TLDR
Expression during microsporogenesis and embryogenesis seems to be a general character of various seed protein genes.
Legumains - a family of asparagine-specific cysteine endopeptidases involved in propolypeptide processing and protein breakdown in plants
TLDR
A recently discovered family of plant and animal cysteine endopeptidases with a cleavage specificity for Asn in the P1 position of peptide bonds that seems to be the result of a co-evolution of enzyme and substrate.
Proteinase A, a storage-globulin-degrading endopeptidase of vetch (Vicia sativa L.) seeds, is not involved in early steps of storage-protein mobilization.
TLDR
The reported data do not agree with the proposed triggering role of proteinase A in storage-globulin breakdown during germination.
Purification, cDNA cloning and characterization of proteinase B, an asparagine-specific endopeptidase from germinating vetch (Vicia sativa L.) seeds.
TLDR
A strong cross-reacting protein band was found in cotyledon extracts of developing seeds, indicating the presence of a very similar enzyme during seed development, and the results of Southern-blot analyses suggested that there are at least two genes for proteinase B.
Redundant Proteolytic Mechanisms Process Seed Storage Proteins in the Absence of Seed-Type Members of the Vacuolar Processing Enzyme Family of Cysteine Proteases
TLDR
It is concluded that seed-type VPEs constitute merely one pathway for processing seed storage protein and that other proteolytic enzymes also can process storage proteins into chains capable of stable accumulation in mature seeds.
Vacuolar Processing Enzymes Are Essential for Proper Processing of Seed Storage Proteins in Arabidopsis thaliana*
TLDR
The overall results suggest that seed-type βVPE is most essential for the processing of storage proteins, and that the vegetative-type VPEs and aspartic proteinase complementβVPE activity in this processing.
Peroxisomal Copper,Zinc Superoxide Dismutase (Characterization of the Isoenzyme from Watermelon Cotyledons)
TLDR
Analysis by reversed-phase high-performance liquid chromatography of the peroxisomal CuZn-SOD incubated with 6 M guanidine-HCI indicated that this enzyme contained a noncovalently bound chromophore group that was responsible for the absorbance peak of the native enzyme at 260 nm.
...
1
2
...