Sedimentation velocity analytical ultracentrifugation and SEDFIT/c(s): limits of quantitation for a monoclonal antibody system.

@article{Gabrielson2007SedimentationVA,
  title={Sedimentation velocity analytical ultracentrifugation and SEDFIT/c(s): limits of quantitation for a monoclonal antibody system.},
  author={John P. Gabrielson and Theodore W. Randolph and Brent S. Kendrick and Michael R. Stoner},
  journal={Analytical biochemistry},
  year={2007},
  volume={361 1},
  pages={
          24-30
        }
}
Sedimentation velocity analytical ultracentrifugation (SV-AUC) has emerged in the biopharmaceutical industry as a technique to detect small quantities of protein aggregates. However, the limits of detection and quantitation of these aggregates are not yet well understood. Although diverse factors (molecule, instrument, technique, and software dependent) preclude an all-encompassing measurement of these limits for the complete system, it is possible to use simulated data to determine the… CONTINUE READING

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