Sectioning of brain tissues.

@article{Paul2008SectioningOB,
  title={Sectioning of brain tissues.},
  author={Carol Ann Paul and Barbara S. Beltz and Joanne E. Berger-Sweeney},
  journal={CSH protocols},
  year={2008},
  volume={2008},
  pages={
          pdb.ip42
        }
}
INTRODUCTIONA fixed brain, although harder than the brain in its natural state, is still not hard enough to cut into very thin slices. To make uniform, thin sections, the brain must be frozen. A wide variety of sectioning devices are available, each with their own requirements. With a microtome, for example, a very sharp knife is drawn manually across a brain that has been frozen to a chilled platform using dry ice. After each cut, the knife is lowered mechanically by a preset amount, giving… CONTINUE READING
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