Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum

@inproceedings{Scheele2013SecretoryPO,
  title={Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum},
  author={Sandra Scheele and Dan Oertel and Johannes Bongaerts and Stefan Evers and Hendrik Hellmuth and K. H. Maurer and Michael Bott and Roland Freudl},
  booktitle={Microbial biotechnology},
  year={2013}
}
Carbohydrate oxidases are biotechnologically interesting enzymes that require a tightly or covalently bound cofactor for activity. Using the industrial workhorse Corynebacterium glutamicum as the expression host, successful secretion of a normally cytosolic FAD cofactor-containing sorbitol-xylitol oxidase from Streptomyces coelicolor was achieved by using the twin-arginine translocation (Tat) protein export machinery for protein translocation across the cytoplasmic membrane. Our results… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 36 references

The Authors. Microbial Biotechnology published by Blackwell Publishing Ltd and Society for Applied Microbiology, Microbial

  • S. Scheele
  • 2012

w-Transaminases for the production of optically pure amines and unnatural amino acids

  • S. Mathew, H. Yun
  • ACS Catal
  • 2012
1 Excerpt

Similar Papers

Loading similar papers…