Secretory production and purification of functional full-length streptavidin from Bacillus subtilis.

@article{Wu2002SecretoryPA,
  title={Secretory production and purification of functional full-length streptavidin from Bacillus subtilis.},
  author={S C Wu and Mohammad Hassan Qureshi and S Wong},
  journal={Protein expression and purification},
  year={2002},
  volume={24 3},
  pages={348-56}
}
Streptavidin is a versatile molecule for many in vitro and in vivo applications. To optimize the production of the full-length streptavidin in a soluble and functional form via secretion using Bacillus subtilis as the expression host, three different strategies were used. These strategies include the construction of a synthetic streptavidin gene, the installation of a transcription terminator, and the use of a sporulation mutant strain. In comparison with the wild-type streptavidin gene in… CONTINUE READING

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