Secretory immunoglobulin A from human milk catalyzes milk protein phosphorylation.

  title={Secretory immunoglobulin A from human milk catalyzes milk protein phosphorylation.},
  author={Georgy A Nevinsky and Kit YYa and Dmitry V. Semenov and Khlimankov DYu and Valentina N Buneva},
  journal={Applied biochemistry and biotechnology},
  volume={75 1},
This article presents evidence that protein kinase activity is an intrinsic property of secretory immunoglobulin A (sIgA) from milk of healthy human mothers. Polyclonal sIgA was purified by sequential chromatography on protein A-Sepharose, DEAE-cellulose, and gel filtration on Toyopearl HW-55 and Sepharose 4B columns. Its purity was established by one- and two-dimensional SDS-PAGE. The protein kinase activity was inhibited by specific antibodies (Abs) against sIgA, and was stable to acidic and… CONTINUE READING


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