Secretory Expression of Nattokinase from Bacillus subtilis YF38 in Escherichia coli

  title={Secretory Expression of Nattokinase from Bacillus subtilis YF38 in Escherichia coli},
  author={Xiaobo Liang and Shifang Jia and Yufang Sun and Meiling Chen and Xiuzhu Chen and Jin Zhong and Liandong Huan},
  journal={Molecular Biotechnology},
Nattokinase producing bacterium, B. subtilis YF38, was isolated from douchi, using the fibrin plate method. The gene encoding this enzyme was cloned by polymerase chain reaction (PCR). Cytoplasmic expression of this enzyme in E. coli resulted in inactive inclusion bodies. But with the help of two different signal peptides, the native signal peptide of nattokinase and the signal peptide of PelB, active nattokinase was successfully expressed in E. coli with periplasmic secretion, and the… 
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