Secretion of recombinant proteins into the culture medium by Escherichia coli and Staphylococcus aureus.

@article{Uhln1989SecretionOR,
  title={Secretion of recombinant proteins into the culture medium by Escherichia coli and Staphylococcus aureus.},
  author={M Uhl{\'e}n and Lars Abrahms{\'e}n},
  journal={Biochemical Society transactions},
  year={1989},
  volume={17 2},
  pages={
          340-1
        }
}
The ability of staphylococcal protein A (SPA) to bind to the Fc part of IgG has been used for the purification of a number of heterologous gene products as fusion proteins. Both the SPA promoter and signal sequence function in Escherichia coli, as well as in a number of Gram-positive bacteria, which facilitates comparisons of the expressed specific products in different hosts. The expression system developed for E. coli yields excretion of the fusion protein to the growth medium, which makes E… 
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The role of oxidative stress in biofilm mutability was investigated by addition of antioxidants to biofilms, and MFs were reduced up to 5-fold, suggesting a role for oxidative damage.
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