Secretion of human serum albumin by Kluyveromyces lactis overexpressing KlPDI1 and KlERO1.

@article{Lodi2005SecretionOH,
  title={Secretion of human serum albumin by Kluyveromyces lactis overexpressing KlPDI1 and KlERO1.},
  author={Tiziana Lodi and Barbara Neglia and Claudia Donnini},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 8},
  pages={4359-63}
}
The control of protein conformation during translocation through the endoplasmic reticulum is often a bottleneck for heterologous protein production. The core pathway of the oxidative folding machinery includes two conserved proteins: Pdi1p and Ero1p. We increased the dosage of the genes encoding these proteins in the yeast Kluyveromyces lactis and evaluated the secretion of heterologous proteins. KlERO1, an orthologue of Saccharomyces cerevisiae ERO1, was cloned by functional complementation… CONTINUE READING
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